Growth and cyanide consumption of <i>P</i>. <i>pseudoalcaligenes</i> CECT5344 wild-type and GntR⁻mutant strains with the jewelry residue as the sole nitrogen source.

<p>Wild-type (filled symbols) and GntR⁻(empty symbols) strains were cultured in minimal media M9 with acetate as carbon source and the cyanide-containing jewelry residue as the nitrogen source. Bacterial growth (triangles) was estimated by protein concentration determination with a modified method of the Lowry procedure [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172908#pone.0172908.ref026" target="_blank">26</a>]. Total cyanide (circles) was determined as indicated in Material and Methods section.