mTOR was involved in the AA-induced activation of PPARγ.

<p>HepG2 cells were cultured in media with 1× and 4× physiological AA concentrations for 48 h. One fraction of the total protein was used to determine the total and phosphorylated levels of the mTOR (A and B), GAPDH, PPARγ (C), and IGF-1 (D) proteins by Western blot analysis. All results contained three replicates (<i>n</i> = 3). The other total protein extracts were analyzed by immunoprecipitation (IP) with anti-PPARγ (E) capture antibodies. Data were expressed as the mean ± SEM. Values with different letters were significantly different (<i>P</i> < 0.05).