<i>In vivo</i> T2* weighted MRI visualizes cardiac lesions in murine models of acute and chronic viral myocarditis - Fig 9

<p>(A) short axis T2*w MRI heart image before (left) (same as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172084#pone.0172084.g008" target="_blank">Fig 8A1</a>) and two days after ferumoxytol injection (right) of a CVB3 infected ABY/SnJ mouse (left, 12 days p.i., right 14 days p.i.) The endogenous MRI contrast due to CVB3 infection is clearly visible prior to ferumoxytol administration. After ferumoxytol administration the T2* hypointense contrast did not increase significantly. It is unclear whether ferumoxytol-containing macrophages or necrotic myocytes or both induce the hypointense contrast in T2*w images observed in Fig A (right). (B) A clear ferumoxytol uptake by macrophages was found 2 days after contrast agent injection and 14 days p.i., as demonstrated by Masson's trichrome (middle row, x12,5 and x200) and Prussian blue (bottom rows, x12,5 and x200) staining.