Binding of D₁/D₅ specific ligand [³H]SCH23390 to hippocampal membranes of wt and <i>Wfs1</i> knockout mice.

<p>Comparison of specific binding of radioligand [³H]SCH23390 to hippocampal membranes of wt and <i>Wfs1</i> knockout mice. (A) Binding curve of [³H]SCH23390 binding to pooled samples of wt (triangle) and <i>Wfs1</i> knockout (circle) mice. The membrane suspensions (3 mg/well) were incubated with different concentrations of [³H]SCH23390 for 60 min and bound radioactivity was measured. Data are presented as mean ± SEM from experiments (n = 3) performed in duplicates. (B) The level of [³H]SCH23390 binding sites of individual wt and <i>Wfs1</i> knockout mice determined in hippocampal membrane suspensions (6.7 mg/ml.) incubated with 4 nM radioligand. Data presented as mean ± SEM of all the mice tested. *P < 0.05. Data of individual mice are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172825#pone.0172825.s006" target="_blank">S1 Table</a>.