PSA reduced IL-1β induced P-c-Jun expression is TLR2 dependent.

<p>P-c-Jun (green color) was detected by immunofluorescent staining in control (A1), TLR2 knockdown (B1) and TLR4 knockdown (C1) H4 cells which were pretreated with or without PSA for 24 hrs and then exposed to IL-1β for 2 hrs The nuclei were counterstained by Dapi (blue), and the cell membrane was stained red. Amplified x600; scale bar = 20 μm; n = 60–70 cells/treatment. The images were representative of three separate experiments with similar results. The analysis (A2, B2 and C2) applied by corrected total cell fluorescence (CTCF) was determined.** P<0.01, ***P<0.001(one-way ANOVA and post-hoc tests).