PLSCR1 amplifies degranulation <i>in vitro</i> in primary culture of BMMC and PCMC.

<p>Wild-type (WT) and <i>Plscr1</i>^<i>-/-</i> (KO) BMMC (A, C, D) and PCMC (B) were sensitized for 24 hours with anti-DNP IgE. After two washes, the IgE-sensitized cells were stimulated with different doses of specific antigen (DNP-HSA) for 30 minutes (A, B) or at different times to the optimal antigen dose of 10 ng/ml (C). Statistical analysis was done by a Two-way ANOVA followed by Sidak’s multiple comparisons test. Data of n independent experiments with n = 12 (A), n = 5 (B) and n = 6 (C) are presented as mean ± s.e.m. *: P <0.05; **: P <0.01; ***: P <0.001. (D) WT and KO BMMC generated in the presence of IL3 and SCF (black bars) or of IL3 alone (open bars) were stimulated for 30 min with 10 ng/ml DNP-HSA. Data of n independent experiments with n = 7 (IL3 + SCF) and n = 3 (IL3) are presented as mean ± s.e.m. Statistical analysis was done by an unpaired Student <i>t</i> test. **: p < 0.01.