Heterologous neutralization responses in two cases of intrasubtype CRF02_AG superinfection.

<p><b>A</b>, <b>B)</b> Table of IC50 values that represent the plasma dilutions (<b>A</b>) or IgG concentrations (<b>B</b>) needed for 50% neutralization of the respective pseudovirus. IC50 values were calculated using nonlinear regression fits of the neutralization curves in GraphPad Prism and are illustrated in a color-coded scheme. Resistance to neutralization was assumed if the plasma or IgG sample could not reach 50% neutralization at the lowest plasma dilution (<1) or highest IgG concentration (>500 μg/mL), respectively, and is indicated in the table with a green shade. Breadth and potency values were calculated as described previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173705#pone.0173705.ref010" target="_blank">10</a>]. Pseudoviruses are all tier 2 covering subtypes A, B, C, G, and CRF02_AG, with the exception of lab strain BaL.26, tier 1, subtype B. MLV was tested as negative control and to ensure absence of ART (<b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173705#pone.0173705.s009" target="_blank">S9 Fig</a>)</b>. Time points filled in with pale red are after superinfection, pale blue after initiation of ART. IC50, breadth, and potency values are calculated using the averages of 2 or more experiments. <b>C)</b> Neutralization curves from plasma samples for NYU6501 & NYU6564 against pseudoviruses T250-4 and Q23.17, shown as the percent neutralization at the reciprocal plasma dilution. <b>D)</b> Neutralization curves from IgG samples for NYU6501 and NYU6564 against pseudoviruses T250-4, shown as the percent neutralization at the given IgG concentration.