Development of bradyzoites <i>in vitro</i> and <i>in vivo</i>.

<p>(A) There was no significant difference in bradyzoite differentiation <i>in vitro</i> across the parasite lines in either tachyzoite conditions (left) (<i>P</i>> 0.99) or bradyzoite conditions (right) (<i>P</i>> 0.99) (Two-way ANOVA).(B) Representative pictures of tachyzoites, partial cysts, and complete cysts produced <i>in vitro</i> as assessed by DBL staining. (C) Brain cyst yields in mice 1–2 months post-infection. All parasite lines produced similar numbers of tissue cysts <i>in vivo</i> (Kruskal-Wallis, ns).