Hypercholesterolemia in AAVmPcsk9-injected, Western diet-fed mice can be reversed by a diet switch and MTPi treatment.

<p><b>A</b>. Experimental design: C57BL/6J mice were i.p. injected once with AAVmPcsk9 and were placed on a Western diet for 16 weeks. At 16 weeks, the baseline group of mice was sacrificed and the remaining were continued with Western diet (progression group) or switched to chow (chow regression group) or MTPi diet (MTPi regression group), for another 6 weeks. <b>B</b>. Plasma levels (mg/dL) of total cholesterol in baseline, progression, chow regression and MTPi regression groups. ***<i>P</i><0.001 when compared with base-line, # p<0.01 when compared with chow regression group. Values are mean±SEM; n = 9–11. <b>C</b>. Fast protein liquid chromatography (FPLC) profile of cholesterol (Chol) and triglyceride (TG) in pooled samples at the end of the experiment. CM/VLDL, chylomicron and very low-density lipoprotein; HDL, high-density lipoprotein; IDL/LDL, intermediate density lipoprotein.