<p>The resulting expression plasmid pIBAINS contains a hybrid protein SOD::INS gene under the control of the <i>deoP1P2</i> promoter. Abbreviations used: Amp<sup>R</sup>, ampicillin resistant; Tet<sup>R</sup>, tetracycline resistant; trpA—transcription terminator trpA; SOD, superoxide dismutase gene; A chain, B chain—chains of human insulin gene; ORI, replication origin.</p
<p>See <i>Plasmid Construction</i> for construct details. Abbreviations used: hph, hygromycin resist...
<p>(A) Schematic representation of the functional units of plasmid pWRG730. The operon containing λ ...
<p>(A) The pSIP411-VP1 plasmid was constructed as described in the article and the purple arrow stan...
<p>The red bars represent the homologous regions between different DNA fragments and vectors. The ye...
<p>Abbreviations used: CG-P, a hybrid promoter carrying the <i>GAL10</i> upstream activating sequenc...
<p>(A) Plasmid pCHRGFP1: pPROBE-NT containing the <i>chr</i> promoter and <i>chrB</i> gene upstream ...
Expression plasmids used We used a plasmid expression system with well established components, close...
Screen and Insert principles and strategies. Configurations of plasmid DNA before and after its inco...
<p>Separate amplification of the 5′ and 3′ ends of the MC58 <i>lpxL1</i> gene, introduction of a nov...
<p>(A) Genetic map of the IncQ-group conjugation plasmid pDSK5191. Protein coding sequences are show...
<p>(<b>A</b>). Schematic showing the process of RCME (Watson 2008): (i) starting with a base strain ...
<p><b>(A)</b>. Schematic representation of the pBHSA plasmid used in our transgenic experiment. pBL ...
<p>(A) One plasmid system. (B) Two plasmid system. In these designs, when Cre recombinase is express...
a<p>In genotype descriptions, the subscripted number refers to a composite deletion and insertion of...
<p>The location of promoters in the plasmids pCM132TC::TE, conferring a Tc<sup>r</sup> phenotype, ar...
<p>See <i>Plasmid Construction</i> for construct details. Abbreviations used: hph, hygromycin resist...
<p>(A) Schematic representation of the functional units of plasmid pWRG730. The operon containing λ ...
<p>(A) The pSIP411-VP1 plasmid was constructed as described in the article and the purple arrow stan...
<p>The red bars represent the homologous regions between different DNA fragments and vectors. The ye...
<p>Abbreviations used: CG-P, a hybrid promoter carrying the <i>GAL10</i> upstream activating sequenc...
<p>(A) Plasmid pCHRGFP1: pPROBE-NT containing the <i>chr</i> promoter and <i>chrB</i> gene upstream ...
Expression plasmids used We used a plasmid expression system with well established components, close...
Screen and Insert principles and strategies. Configurations of plasmid DNA before and after its inco...
<p>Separate amplification of the 5′ and 3′ ends of the MC58 <i>lpxL1</i> gene, introduction of a nov...
<p>(A) Genetic map of the IncQ-group conjugation plasmid pDSK5191. Protein coding sequences are show...
<p>(<b>A</b>). Schematic showing the process of RCME (Watson 2008): (i) starting with a base strain ...
<p><b>(A)</b>. Schematic representation of the pBHSA plasmid used in our transgenic experiment. pBL ...
<p>(A) One plasmid system. (B) Two plasmid system. In these designs, when Cre recombinase is express...
a<p>In genotype descriptions, the subscripted number refers to a composite deletion and insertion of...
<p>The location of promoters in the plasmids pCM132TC::TE, conferring a Tc<sup>r</sup> phenotype, ar...
<p>See <i>Plasmid Construction</i> for construct details. Abbreviations used: hph, hygromycin resist...
<p>(A) Schematic representation of the functional units of plasmid pWRG730. The operon containing λ ...
<p>(A) The pSIP411-VP1 plasmid was constructed as described in the article and the purple arrow stan...