Additional file 2: of Development of a test that measures real-time HER2 signaling function in live breast cancer cell lines and primary cells

Figure S1. The PI3K/AKT pathway significantly contributes to the ligand-driven HER2 signaling activities detected by CELx HSF tests. (A and B) SKBr3 cells were seeded in sensor plates and then treated with a serial titration of the PI3K/AKT pathway inhibitor GDC-0941 (0 nM to 2700 nM) two hours prior to maximal stimulation with NRG1b (800 pM) (A) or EGF (600 pM) (B). CELx curves are displayed using Baseline Delta CI values. The relative CELx signals were baseline subtracted to the time point (arrow) when the stimulus (EGF or NRG1b) was added and the signals induced by stimulus alone without the drug (GDC-0941) were set as baselines. Dose–response curves of GDC-0941 inhibition on NRG1b and EGF-driven HER2 signals are shown in the insets. (PDF 92 kb