Effects of Utx deficiency on gene expression in mESCs and adipocytes.

<p>(A) Heat map visualizations of the 79 genes that are differentially expressed in <i>Utx</i>-deficient and control mESCs and adipocytes. Two clones were used for each group. Data for the heat maps are normalized using average linkage clustering on entities and represent median-centered log-transformed values. Red and blue correspond to high and low expression, respectively, compared with the experiment-wide median. (B) mRNA levels of <i>Mgp</i> and <i>Dcn</i> normalized to <i>β-actin</i>. The experiments were performed independently with three clones, and the results are expressed as mean ± SE (n = 3); *p<0.05. (C) GSEA enrichment score curves described with two sets of adipocytes from the Molecular Signatures Database (MSigDB). The graph at the bottom of each panel represents the ranked, ordered, non-redundant list of genes. Vertical black lines indicate the position of genes from the studied gene set in the ordered, non-redundant data set. The green curve corresponds to the enrichment score (ES) curve, which is the running sum of the weighted enrichment score obtained with the GSEA software. NES, normalized enrichment score: FDR, false discovery rate; NOM, nominal. (D, E) mRNA levels of <i>c-Myc</i> normalized to <i>β-actin</i> in (D) mESC and adipocytes, (E) mESCs, EBs, and RA-induced differentiation. The experiments were performed independently with three clones, and the results are expressed as mean ± SE. (n = 3) *p<0.05. (F) mRNA levels of <i>aP2</i> normalized to <i>Gapdh</i> in <i>Utx</i>-deficient or control mESCs and adipocytes in the presence of 10058-F4. The representative result is shown as mean ± SE. (n = 3) *p<0.05.