miR-181b regulates vascular stiffness age dependently in part by regulating TGF-β signaling - Fig 4

<p><b>A, Rate of nitric oxide (NO) production from the thoracic aorta of miR-181a1/b1^-/- (a/b KO) (n = 22) and control (WT) (n = 20).</b> Data are shown normalized to baseline WT aorta. <b>B</b>, Western blot of phosphorylated eNOS (p-eNOS) in the aorta of miR-181a1/b1^-/- (a/b KO) and control (WT) (p = 0.010, n = 6). <b>C</b>, Phenylephrine dose response (1nm-10uM) of the denuded thoracic aorta from miR-181a1/b1^-/- (a/b KO) and control (WT) (n = 4–5). <b>D</b>, Phenylephrine dose response (1nm-10uM) of the thoracic aorta treated with L-NAME (200nM) from miR-181a1/b1^-/- (a/b KO) and control (WT) (n = 5). Data are presented as % constriction from maximum constriction at 10uM. <b>E</b>, Dose response relaxation to acetylcholine (1nM-10uM) in denuded thoracic aorta and <b>F</b>, aorta pretreated with L-NAME (200nM) from miR-181a1/b1^-/- (a/b KO) and control (WT). The vessels were pre-constricted with phenylephrine (1uM). Values are mean ±SEM, *p<0.05, **p<0.01, ***p<0.001.