Identification of MCPIP1 target regions within the 3'UTR of C/EBPβ mRNA.

<p><b>A</b>. Schematic representation of genetic constructs used in luciferase activity assays. <b>B</b>. Luciferase assay of HepG2 cells transfected with a pmir-GLO reporter vector carrying a relevant fragment of the 3'UTR of C/EBPβ mRNA linked to a coding sequence of firefly luciferase and pcDNA3.1 expression vector coding for wild type MCPIP1 or MCPIP1 with abolished RNase activity (MCPIP1-D141N). Empty pcDNA3.1 vector served as a control. All values were normalized to the same external reference i.e. value measured for cells co-transfected with empty luciferase-expressing vector and an empty pcDNA3.1 expression vector. Graph shows mean ± SD n = 3; *p<0.05, **p <0.02 ***p<0.001, ns—not significant (Student-t test).