Analysis of e11.5 <i>Kif3a</i> conditional mutants.

<p>Frontal view of e11.5 (A) wild-type, (B) <i>Kif3a</i>^<i>f/f</i><i>;Wnt1-Cre</i>, (C) <i>Kif3a</i>^<i>f/f</i><i>;Crect</i>, and (D) <i>Kif3a</i>^<i>f/f</i><i>;AP2-Cre</i> embryos. PNA staining in e11.5 sections of (E) wild-type, (F) <i>Kif3a</i>^<i>f/f</i><i>;Wnt1-Cre</i>, (G) <i>Kif3a</i>^<i>f/f</i><i>;Crect</i>, (H) <i>Kif3a</i>^<i>f/f</i><i>;AP2-Cre</i> embryos. PHH3 staining in e11.5 frontonasal sections of (I) wild-type (n = 15), (J) <i>Kif3a</i>^<i>f/f</i><i>;Wnt1-Cre</i> (n = 12), (K) <i>Kif3a</i>^<i>f/f</i><i>;Crect</i> (n = 12), (L) <i>Kif3a</i>^<i>f/f</i><i>;AP2-Cre</i> (n = 19) embryos. CC3 staining in e11.5 frontonasal sections of (M) wild-type (n = 14), (N) <i>Kif3a</i>^<i>f/f</i><i>;Wnt1-Cre</i> (n = 17); (O) <i>Kif3a</i>^<i>f/f</i><i>;Crect</i> (n = 12), (P) <i>Kif3a</i>^<i>f/f</i><i>;AP2-Cre</i> (n = 13) embryos. PHH3 staining in e11.5 palatal sections of (Q) wild-type (n = 10), (R) <i>Kif3a</i>^<i>f/f</i><i>;Wnt1-Cre</i> (n = 20), (S) <i>Kif3a</i>^<i>f/f</i><i>;Crect</i> (n = 12), (T) <i>Kif3a</i>^<i>f/f</i><i>;AP2-Cre</i> (n = 17) embryos. CC3 staining in e11.5 palatal sections of (U) wild-type (n = 12), (V) <i>Kif3a</i>^<i>f/f</i><i>;Wnt1-Cre</i> (n = 20); (W) <i>Kif3a</i>^<i>f/f</i><i>;Crect</i> (n = 13), (X) <i>Kif3a</i>^<i>f/f</i><i>;AP2-Cre</i> (n = 16) embryos. (Y-CC) Quantification of measured values represented as box plots where the median and interquartile range are displayed. Red dots on the graph represent <i>Kif3a</i>^<i>f/f</i><i>;Crect</i> mutants that did not display a craniofacial phenotype and were not included in the rest of the analyses (2/10). For each genotype, <i>n = 3</i> embryos were utilized for PHH3/CC3 analyses. P-values: (*) 0.05≥P>0.01; (**) 0.01≥P>0.001; (***) P≤0.001. Scale bars: (A-D) 500 μm, (E-H) 200 μm, (I-P) 225 μm, and (Q-X) 350μm.