Effect of an olive-oil bioactive extract on basal TEER (A) and TNF-α induced decrease in TEER (B) <i>in vitro</i>.

<p>Caco-2/TC-7 cells were allowed to differentiate on permeable filters in the presence of an olive-oil bioactive extract (OBE; 100 μg/mL), genistein (50 μmol/L; positive control), and DMSO (0.1% v/v; vehicle control [C]) for 5 d. (A) Barrier integrity was assessed by measuring the TEER over time. To investigate potential protective effects of OBE on Caco-2/TC-7 barrier integrity, TNF-α (100 ng/mL) was added to the basolateral side for 24 h on d 5 to induce monolayer disruption. (B) TEER was measured before and after TNF-α administration and values were normalized to the untreated control. Bars represent means of 3 individual experiments ± SEM. Different letters indicate significant differences among treatments (<i>p</i> < 0.05).