Construction of Cyp2b9/10/13-null mice.

<p><b>(A)</b> Cyp2b9/10/13-null mice were produced using Crispr/Cas9 with sgRNA target sites for all three genes. A 287 kb deletion mutant was produced that lacks these three Cyp2b genes found in tandem repeat on chromosome 7. <b>(B)</b> Two mice heterozygote for a chromosome deletion lacking the three Cyp2b genes (Cyp2b10, 2b13, 2b9) in tandem repeat were produced. PCR confirmation of the 287kb deletion from the first null mice produced is shown in lanes 4 and 21 using the F2/R2 primer combination that produces a 1066 bp fragment. <b>(C)</b> Subsequent breeding produced mice lacking Cyp2b9/10/13 as demonstrated by the presence of the 1066 bp PCR product. <b>(D)</b> Heterozygotes were discerned from homozygotes by PCR of Cyp2b13 indicating a heterozygote.