SIRT6 activates the NF-κB/p65, p38-MAPK and ERK signaling pathways and thus affects cellular mitochondrial energy metabolism in DMCs.

<p>(A) A western blot analysis of the <i>Sirt6</i> KO group showed enhanced activation of the NF-κB/p65, p38-MAPK, and ERK signaling pathways on phosphorylation of those signaling molecules. Quantitative analysis obtained the same relative results. (B) PCR array analysis showed a significant difference between the KO and WT group with respect to the mRNA expression levels of Atp4b and Cox6a2. They were reduced by 51% and 54% in the KO group, whose ATP levels in the DMCs were significantly lower than those of the WT group. The gene expression level was normalized to GAPDH (n = 3). *<i>p</i><0.05 versus CO. CO = control. GAPDH was used as a control.