Extraneural inoculation of DY TME fails to establish infection in hamsters.

<p>Western blot analysis of proteinase K digested brain material from hamsters infected with either the HY or DY TME agents by various routes of inoculation. A) Inoculation of hamsters with the HY TME agent results in development of disease and detection of PrP^Sc. B) Hamsters inoculated with DY TME a single (A) or multiple (B) times only develop disease following direct inoculation of the CNS. The 19 and 21 kDa unglycoslyated PrP^Sc polypeptides are indicated on the left of the panel. i.c., intracerebral; i.p., intraperitoneal; p.o., per os; e.n., extranasal; i.v., intravenous.