Effect of luteolin and kaempferol on the expression levels of ANO1 in PC-3 cells.

<p>A) Immunoblot analysis of ANO1 in PC-3 cells expressing high levels of ANO1. Cells were incubated with indicated concentration of luteolin and kaempferol for 24 hour. B) The ANO1 band intensity was normalized to β-actin (mean ± S.E., n = 3–4). C) Effect of luteolin and kaempferol on gene expression levels of ANO1 in the PC-3 cells (mean ± S.E., n = 3). D) Luteolin-induced time-dependent changes of ANO1 protein levels in the PC-3 cells. E) The ANO1 band intensity was normalized to β-actin (mean ± S.E., n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.