Induction of [<i>PSI</i>⁺] prion formation by overexpression of Sup35.

<p><b>A.</b> Fluorescence micrographs are shown for [<i>PIN</i>⁺][<i>psi</i>^-] versions of wild-type, <i>abp1</i>, <i>crn1</i> and <i>pan1</i> mutant strains containing the Sup35NM-GFP plasmid induced with copper for 24 hours. Representative Images are shown for rod/ribbon-like and foci aggregate formation. The numbers indicate the percentage of cells containing each type of aggregate from an average of 300 cells counted. The scale bar is 3μm. <b>B.</b> Western blot analysis of the wild-type and <i>abp1</i> mutant strain following induction of Sup35NM-GFP. Blots were probed with αSup35 or α-Pgk1 as a loading control <b>(C)</b> [<i>PSI</i>⁺] prion formation was quantified in the wild-type and <i>abp1</i> mutant strain containing the <i>Sup35NM-GFP</i> plasmid following 24 h of copper induction. Data shown are the means of three independent biological repeat experiments expressed as the number of colonies per 10⁴ viable cells. Error bars denote standard deviation; * marks statistical significance at p<0.01.