BR activities of iso-carbaBL and 6-deoxoBL.

<p>(a) Chemical structures of brassinolide (BL), castasterone (CS), iso-carbaBL, and 6-deoxoBL. (b) Wild-type (WT) plants were grown in an MS agar medium containing 3% sucrose at the indicated concentrations of BL or iso-carbaBL for 7 d, after which root length was measured. Data are represented as means ± SE (n > 14, **<i>p</i> < 0.01, Tukey-Kramer test. The difference was indicated by alphabetical order) (c) BR activities of BL, CS, 6-deoxoBL, and iso-carbaBL in Arabidopsis were analysed. <i>det2-1</i> plants were grown for 8 d in the dark, after which hypocotyl length was measured. Data are represented as means ± SE (n = 15). (d) BR activities of BL, CS, iso-carbaBL and 6-deoxoBL in rice were analysed. WT plants were grown for 4 d under continuous light and treated with BRs for another 2 d, after which the angle of the lamina joint was measured. Data are presented as means ± SE (n = 10). (e) Response to 1 μM BRs in Arabidopsis. The white triangles indicate the border of hypocotyl and root. (f) Lamina joint angle in response to 100 ng BRs. The experiments were repeated twice with similar results.