Small RNA sequencing results.

<p>(A) Read length (mean ± SEM) distribution plot for all samples constructed using raw reads. (B) Barplot showing total, mapped and miRNA reads for each plasma sample. Sample P60_17 generated very few miRNA reads, however it was included because it clustered normally in subsequent principal component analysis (PCA). (C) PCA plot using transformed normalised data and showing partial separation between Day 0 and Day 60 samples. (D) Volcano plot showing differences in individual miRNA values between Days 0 and 60 of pregnancy, using transformed normalised data. Values with fold-change > 2 and FDR < 0.05 are highlighted in red.