Gr-1⁺ cells are required for TNF and IL-12 production early during pulmonary infection with <i>L</i>. <i>pneumophila</i>.

<p>B6 mice treated with isotype control (ISO) or anti-Gr-1 (α-Gr-1) antibody were infected with Δ<i>flaA L</i>. <i>pneumophila</i>. Levels of IL-1α (A), IL-1β (B), IL-6 (C), TNF (D), IL-18 (E), IL-12p70 (F), IL-12p40 (G), IL-4 (H), and IL-10 (I) in the BALF at 24 and 72 hours post-infection were quantified by ELISA. Data shown are the pooled results of 2 independent experiments with 3 or 4 mice per group per experiment. * is p<0.05, ** is p<0.01, and *** is p<0.001 by unpaired t-test. NS is not significant. Dashed line represents the limit of detection.