EMBS does not induce autophagy.

<p>(A) Autophagy was measured by determining the levels of LC3B II by western blot. Cells were treated with DMSO as control (lane 1), bafilomycin A1 (lane 2) as positive control or EMBS for 4 (lane 3), 10 (lane 4), 14 (lane 5) or 24 (lane 6) h. Total protein loading was measured by western blot against actin. The graph represents expression as a percentage of the positive control that represents cells exposed to bafilomycin A1. Both blot and graph are representative of three independent experiments.