Proposed applications of the platform and an example.

<p>(a) Proposed applications of the platform containing an embryo (3D) with arrayed microchannels (2D). First, blood vessels with blood flow are induced into a few channels arranged on the lateral-side surface from the surface of an egg yolk, a nutrient source. Second, local stimulation of the circulatory system of the chick embryo can easily be achieved by channel-specific injection. (b) As an application example, we fabricated five culture chambers on a patterned lateral side surface and designed the areas of blood vessel formation for each chamber based on our design theory. Next, small holes (φ1.5 mm) were opened on the outer membrane without leakage, and water-based ink imitating drug was injected into each chamber. The release of ink within chamber was observed clearly. Because the culture chamber was an enclosed area, the injected ink was confined within each chamber without immediately influencing the other parts of the CAM. In addition, different drugs can be injected into arrayed micro-channels and comparison of drug delivery by local blood vessels is possible.