Survival rate of embryos and the penetration process of CAM with blood vessels.

<p>(a) The survival rate of embryos was investigated using cubic eggshells with a patterned surface containing a 15×15 mm² air chamber with a 2-mm inducing channel. The survival rate was about 70% until day 18. (b) Typical time lapse images of blood vessel development. On day 3, induced blood vessels were not observed, and on day 7, accompanied with the rapid development of the extraembryonic circulatory system, blood vessels started to penetrate into the inducing channel. The increasing tendency of penetration was observed until about day 15. (c) Areas of blood vessel formation over time for the same cubic eggshell from day 3 to day 21 (N = 6 samples).