Cytotoxic steroid derivatives from the Vietnamese soft coral <i>Sinularia brassica</i>

<p>Using various chromatographic separations, six ergostane-type steroids, including one new compound sinubrassione (<b>1</b>), and two pregnene-type steroid glycosides, including one new compound sinubrassioside (<b>7</b>), were isolated from methanol extract of the Vietnamese soft coral <i>Sinularia brassica</i>. The structure elucidation was confirmed by spectroscopic methods including 1D, 2D NMR and HR-ESI-MS. The cytotoxic activities of all the isolated compounds against three human cancer cell lines were also evaluated using MTT-based colorimetric assays.