Exploring Proteomic Variation in Pacific Oysters (Crassostrea gigas)

150 sibling Pacific oysters (<i>Crassostrea gigas</i>) were outplanted at five different sites around Puget Sound. Oysters were placed in exclusion cages either inside or outside of eelgrass beds. After a month of environmental exposure, gill tissue samples were collected for proteomic analyses. Initial DIA mass spectrometry methods on a subset of oysters (one per site and eelgrass condition, 10 total) provide data for upwards of 6,000 proteins. A subset of stress-related proteins reveal site-specific expression patterns