Analysis of <i>pin1</i> mRNA levels and distribution during zebrafish development.

<p>(A) <i>pin1</i> mRNA levels were monitored by RT-qPCR on cDNA from embryos of the indicated stages: 1–2 cell (0 hpf), dome (4:30 hpf), shield (6 hpf), 2 somites (10:30 hpf), 20 somites (19 hpf),1 dpf, 2 dpf, 3 dpf, 4 dpf and 6 dpf. Data was normalized using elongation factor 1<i>α</i>(<i>ef1α</i>) and ribosomal protein L13a (<i>rpl13a</i>) mRNA levels as internal controls and is shown as relative abundance comparing with 1–2 cell stage. (B) Analysis of <i>pin1</i> mRNA levels by semi quantitative RT-PCR: 1-2-cell (0 hpf), 64-cell (2 hpf), high (3:30 hpf), sphere (4 hpf) and 40%-epiboly (5 hpf). As an internal control, <i>ef1α</i>was amplified on the same cDNA samples. Signal intensity was quantified using ImageJ software and expressed relative to 1–2 cell stage. (C) <i>pin1</i> mRNA distribution in whole embryos at the indicated developmental stages analysed by in situ hybridization (100 x magnification). (D) <i>pin1</i> mRNA pattern in the head at prim-5 (24 hpf) and long-pec (48 hpf) stages. Left: dorsal, center: lateral, right: frontal (200 x magnification). hpf, hours post-fecundation, dpf, days post-fecundation. T: telencephalon, D: diencephalon, C: cerebellum.