Analysis of Pin1 isovariants in zebrafish extracts.

<p>(A) Representative images of two-dimensional (2D) western blot analysis of zebrafish extracts from 4:30 and 24 hpf embryos probed with anti-Pin1. Left and right panels exhibit different exposure times of the same immunoblot. (B) Left panels: magnification of the immunoblots shown in (A). Pin1 isovariants are numbered from the most basic (1) to the most acidic (4). Right panels: 2D western blot analysis of zebrafish extracts from 4:30 and 24 hpf embryos treated with λ phosphatase. pI: isoelectric point, MW: molecular weight.