Additional file 1: of Cripto-1 acts as a functional marker of cancer stem-like cells and predicts prognosis of the patients in esophageal squamous cell carcinoma

Supplementary materials and methods, including Quantitative RT-PCR, Western blotting, Flow cytometry, Silencing CR-1 with shRNA, and Immunofluorescence staining. Table S1. Primer sequences for qRT-PCR assay. Table S2. shRNA sequences targeting CR-1. Table S3. Incidence of tumor formation in nude mice injected with ESCC cells. Table S4. The frequency of lung metastasis (1x104 cells/mouse). Table S5. Significant difference of CR-1 expression between carcinoma and adjacent normal tissues. Figure S1. The levels of CR-1expression and the silencing efficiency of CR-1 shRNA in ESCC cells. Figure S2. Silencing CR-1 expression significantly represses the self-renewal and tumorigenicity inTE-1 cells. Figure S3. Suppression of CR-1 expression inhibits the invasive and metastatic capabilities of TE-1 cells in vitro and in vivo. Figure S4. The effect of silencing CR-1 on the expression of MMPs in EC109 cells. (DOC 1602Â kb