MOESM1 of Molecular mechanisms by which HERV-K Gag interferes with HIV-1 Gag assembly and particle infectivity

Additional file 1: Fig. S1. Coexpression of HERV-K GagPro reduces HIV-1 release efficiency and infectivity of released particles. HeLa cells were cotransfected with HIV-1 pNL4-3 and indicated plasmids encoding non-Flag-tagged Gag at different ratios (A) and at 10:1 ratio (C). The amount of p24 was measured as described in Fig. 3c. P values, compared with HERV-K Gag, were determined using a Student’s t test. *, P < 0.01; **, P < 0.001; ***, P < 0.0001; n.s., not significant. (B) Cell and viral lysates from cotransfected cells were subjected to SDS-PAGE and analyzed by immunoblotting with HIV-Ig or anti-HERV-K Gag antibody. (D) The viruses were recovered from supernatants by ultracentrifugation and normalized by p24 ELISA. TZM-bl cells were infected with the recovered viruses. At 2 days post-infection, luciferase activities were measured by luminometor. Data from three independent experiments are shown as means ± standard deviations. P values were determined using a Student’s t test. *, P < 0.01; **, P < 0.001; ***, P < 0.0001; n.s., not significant