KBP and Kif15 function in efficient chromosome alignment.

<p><b>(A)</b> Scheme outlining the experimental set-up of the live-cell imaging. <b>(B)</b> Quantification of prometaphase duration as observed in the live-cell imaging in control (C), KBP-, Kif15- or KBP/Kif15-silenced cells. Prometaphase was defined as the time needed for cells to progress from NEBD till anaphase onset. Time points are represented in box-and-whisker plots. Boxes show the upper and lower quartiles (25–75%) with a line at the median, whiskers extend from the 5^th to the 95^th percentile. Dots represent the outliers (data were compared using Mann-Whitney <i>U</i> tests, ** means <i>p</i> ≤ 0.01, **** means <i>p</i> ≤ 0.0001). <b>(C)</b> Representative stills from the live-cell imaging of control, KBP-, Kif15- or KBP/Kif15-silenced cells. Arrowheads highlight chromosomes that show less efficient alignment to the metaphase plate. Time is indicated in the upper right corner as hh:mm. Scale bars represent 5μm.