Delayed histochemical alterations within the neurovascular unit due to transient focal cerebral ischemia and experimental treatment with neurotrophic factors - Fig 5

<p><b>Exemplified fluorescence staining of collagen IV (Coll), GFAP and Iba depending on the region with respect to the primary ischemic lesion, i.e. the ischemic core, the ischemic border zone and a control area located at the contralateral, non-affected hemisphere (A) and respective quantification in the overall sample (B).</b> A strong immunolabeling was found for collagen IV and Iba at the ischemic core, diminishing towards the border zone and the control region, while most impressive GFAP-immunostaining was seen at the ischemic border zone in terms of a glial scar formation. Quantitative analyses confirmed the qualitative findings in terms of a most prominent up-regulation of immunoreactivities for collagen IV and Iba in the ischemic core, while the strongest immunoreactivity for GFAP was seen at the ischemic border zone. Scale bar: in A (also valid for all other micrographs) = 75 μm. Bars represent means and added lines represent the standard error of means. *, p < 0.05; **, p < 0.01; ***, p < 0.001.