Analysis of bone marrow progenitors in <i>booreana</i> (<i>boo/boo</i>) and WT mice.

<p>Bone marrow from adult mice was prepared and stained with antibodies to distinguish hematopoietic progenitors flow cytometrically. A lineage cocktail of antibodies was used to gate Lin^- subsets, and Sca-1 and c-kit staining used to identify the Lin^-c-kit⁺Sca-1⁺ (LSK) subset. Staining for Flt3 and CD150 was used to distinguish LT-HSCs and MPPs, and staining for IL-7R and Flt3 was used to distinguish CLPs. Propidium iodide (PI; 1 μg/ml) staining was used to gate live cells as PI^-. Gates were set on bivariate plots using isotype control antibodies, and numbers in gates reflect % positive cells amongst Lin^-PI^- bone marrow cells. <b>(A)</b> Staining profiles for representative individual mice are shown. <b>(B)</b> Percent cells amongst viable Lin^- bone marrow is shown for 3 individual mice, with mean shown by a bar and statistically significant results (p ≤ 0.05) boxed in red.