Binding of TCRm antibodies to p53RMP/HLA-A2 complexes on live cells detected by flow cytometry.

<p>(<b>A</b>) The left panel illustrates purified T1-29D mAb staining of T2 cells pulsed with p53RMP peptide as well as 2 other p53 peptides and peptides derived from survivin, HCMV and Flu (at 100μM). Staining was detected using an APC-conjugated anti-mouse secondary antibody. The T1-84C supernatant was tested only against Flu and p53RMP peptides (right panel). (<b>B</b>) T2 cells pulsed with the p53RMP peptide at 100μM were stained with the purified T1-29D mAb at the indicated concentrations. The HLA-A2 specific mAb BB7.2 was used in parallel to detect HLA-A2 expression. Mean fluorescence intensities (MFIs) of the staining were plotted on the right panel (for clarity the left panel does not contain all of the tested concentrations). (<b>C</b>) T2 cells pulsed with the p53RMP peptide at various concentrations were stained with T1-29D and BB7.2 at 10 μg/ml. MFIs of the staining were plotted on the right panel. Flu peptide pulsing at 100μM was used as a control. Data in each panel is representative of two independent experiments.