Dendritic cell viability following high (300μM), medium (150μM) and, low (75μM) dose PA treatment.

<p>MoDCs were inoculated with PA in a 2:1 molar ratio with BSA for (A) 12 hrs, (B) 24 hrs, or (C) 48 hrs. The cells were analyzed for loss of viability by flow cytometry using Annexin V and propidium iodide (PI) staining. LPS was used as a positive control to assess MoDC activation. The data is represented as the percent of total MoDCs negative for both Annexin V and PI normalized to the BSA negative control. (D) PA induces caspase 3/7 activity. MoDCs were treated with PA complexed 2:1 with BSA for 36 hrs. Caspase 3/7 activity was measured by the emitted fluorescence of cleaved DEVD-AMC. N = 3. Statistical analysis was calculated based on One way ANOVA (** = p<0.01, *** = p<0.001, **** = p<0.0001).