Cells expressing <i>dpb11-m1</i>,<i>2</i>,<i>3</i>,<i>5</i>,<i>ΔC</i> exhibit a defect in cell growth and DNA replication.

<p>(A,B) Ten-fold serial dilutions of <i>dpb11-td</i> cells expressing <i>DPB11</i>-wild type, vector only control, <i>dpb11-ΔC</i>, <i>dpb11-m1</i>,<i>2</i>,<i>3</i>,<i>5</i>, or <i>dpb11-m1</i>,<i>2</i>,<i>3</i>,<i>5</i>,<i>ΔC</i> at the (A) permissive conditions (CSM-Ura, 30°C) or (B) restrictive conditions (CSM-Ura+gal+doxycycline, 37°C). (C) Western analysis of whole cell extracts of <i>dpb11-td</i> cells expressing vector, <i>DPB11</i>-wild type, <i>dpb11-ΔC</i>, <i>dpb11-m1</i>,<i>2</i>,<i>3</i>,<i>5</i>, or <i>dpb11-m1</i>,<i>2</i>,<i>3</i>,<i>5</i>,<i>ΔC</i> under restrictive conditions. (D) Experiments similar to those in (C) were averaged and plotted. Bands from Western experiments were quantified with Odyssey software as described in Materials and Methods. (E) FACS analysis was performed as described in Materials and Methods on <i>dpb11-td</i> cells expressing <i>DPB11</i>-wild type, vector only control, <i>dpb11-ΔC</i>, <i>dpb11-m1</i>,<i>2</i>,<i>3</i>,<i>5</i>, or <i>dpb11-m1</i>,<i>2</i>,<i>3</i>,<i>5</i>,<i>ΔC</i> under restrictive conditions. Cells were synchronized in G₁ with s were synchronized in Gl, D) Experiments similα-factor for the time points indicated.