TRPM8 expression and function in TRG neurons.

<p>(A) Distribution of TRPM8 in the trigeminal nucleus caudalis, trigeminal ganglia and skin. Nuclei in the ganglia where stained with DAPI. (B) Averaged current traces during the bath application of menthol (100μM) in male and female TRPM8 expressing TRG neurons. Bath temperature was 22°C. N = 20 male neurons from 4 mice, N = 32 female neurons from 7 mice. (C) Comparison of the peak currents from B. Asterisk indicates p = 0.0066, t-test. (D) Averaged holding currents (holding potential -60mV) in female and male TRPM8 TRG neurons during bath cooling. N = 14 female neurons from 3 mice, N = 11 male neurons from 3 mice. (E) Averaged holding currents (holding potential -60mV) versus temperature relationships for the data in D.