The role of <i>vadA</i> in conidia.

<p><b>(A)</b> Viability of the conidia of WT (FGSC4), Δ<i>vadA</i> (THS33.1), and C’ (THS34.1) strains grown at 37°C for 5 and 10 days (** P < 0.01). <b>(B)</b> Amount of trehalose per 10⁷ conidia in the two-day-old conidia of WT (FGSC4), Δ<i>vadA</i> (THS33.1), and C’ (THS34.1) strains (measured in triplicate) (*** P < 0.001). <b>(C)</b> Tolerance of the conidia of WT (FGSC4), Δ<i>vadA</i> (THS33.1), and C’ (THS34.1) strains to H₂O₂ treatment. <b>(D)</b> Amount of β-glucan (pg) per 10³ conidia in the two-day-old conidia of WT (FGSC4), Δ<i>vadA</i> (THS33.1), and C’ (THS34.1) strains (measured in triplicate). <b>(E)</b> Levels of <i>vadA</i>, <i>brlA</i>, <i>wetA</i>, <i>tpsA</i>, <i>orlA</i>, <i>rodA</i>, and <i>fksA</i> transcripts in the conidia of WT (FGSC4), Δ<i>vadA</i> (THS33.1), and C’ (THS34.1) strains. Equal loading of total RNA was confirmed by ethidium bromide staining of rRNA.