Developmental phenotypes of the Δ<i>vadA</i> mutant.

<p><b>(A)</b> Colony photographs of WT (FGSC4), Δ<i>vadA</i> (THS33.1), and the complemented (THS34.1) strains point inoculated on solid MM and grown for four days (top and bottom panels). The bottom panel shows close-up views of the center of the plates. (bar = 0.5 mm) <b>(B)</b> Quantitative analysis of conidiospore formation of the strains shown in (A). <b>(C)</b> Northern blot for <i>brlA</i>, <i>vosA</i>, and <i>velB</i> mRNAs in WT (FGSC4) and Δ<i>vadA</i> (THS33.1) strains in vegetative growth (Veg) and post-asexual developmental induction (Asex). Numbers indicate the time (h) of incubation after induction of asexual development. Equal loading of total RNA was confirmed by ethidium bromide staining of rRNA. <b>(D)</b> Colony photographs of WT (FGSC4), Δ<i>vadA</i> (THS33.1), and the complemented (THS34.1) strains point inoculated on solid SM and grown for five days (top and bottom panels). The bottom panel shows close-up views of the center of the plates. (bar = 0.5 mm) <b>(E)</b> Quantitative analysis of cleistothecia formation of strains shown in (A) (* P < 0.05).