Temporal analysis of the response to 2 Gy within distinct subdomains of the lateral ventricle.

<p>(A-D) Adult mice were exposed to 2 Gy and the percentage of Ki67⁺ (panel A), Glial Fibrillary Acidic Protein (GFAP)⁺ (panel B), GFAP⁺Ki67⁺ (panel C), and doublecortin (Dcx⁺) (panel D) cells assessed in the ventral or dorsolateral subdomains as indicated. (E-F) The quantification (panel E) of the percentage of GFAP⁺Ki67⁺ cells in all subdomains and representative images (panel F) at 7 days after 2 Gy. (G-H) The quantification (panel G) of the percentage of Dcx⁺Ki67⁺ cells amongst the Dcx⁺ cells in the ventral and dorsolateral subdomains of unirradiated control mice and at 7 and 14 days post 2 Gy ionising radiation (IR) and representative images (panel H). Experiments were carried out on 3-month-old mice and results represent the mean ± SEM of <i>n</i> ≥ 3 mice for each condition. Scale bars, 25 μm. Student <i>t</i> test was performed relative to the control group, * <i>P</i> < 0.05, ** <i>P</i> < 0.01, *** <i>P</i> < 0.001, ns = not significant. Underlying data can be found in the <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2001264#pbio.2001264.s007" target="_blank">S1 Data</a> file.