Reproductive performance of females mated to EcR or USP knockdown males over period of 10 days.

<p>Panel A represents the overall fecundity (total no. of eggs laid/10 days) of mated females while Panels B and C represent day wise fecundity (no. of egg laid/day from day1-10) of mated females. The overall fecundity of EcR knockdown mates was significantly lower compared to controls (***<i>p</i><0.0001). However, overall fecundity of USP knockdown mates was comparable to their controls. (<i>p</i> = 0.08). Further, EcR knockdown mates laid eggs for 24hr ASM, albeit at significantly fewer numbers (***<i>p</i><0.0001) when compared to control and did not lay eggs from days 2–10. USP knockdown mates did not deviate from controls on day wise egg laying. Interestingly, there were no progeny from the eggs laid by EcR knockdown mates over period of 10 days (overall fertility, ***<i>p<</i>0.0001, Panel D; day-wise fertility, ***<i>p<</i>0.0001 Panel E). USP knockdown or control mates had comparable overall (Panel D; <i>p =</i> 0.3, USP) as well as day wise fertility (Panel F; <i>p =</i> 0.06 lowest) EcR control and knockdown mates differ significantly on total % hatchability (Panel G; EcR, ***<i>p</i><0.0001) as well as % hatchability on day 1 (Panel H; ***<i>p<</i>0.0001). USP control and knockdown mates had significant differences in % hatchability on days 8 and 10 (Panel I; <i>p</i> = 0.01) but that did not have a significant bearing on the overall % hatchability (Panel H; <i>p</i> = 0.3). Values given here are Mean±SEM involving at least 15–30 females depending on the hormone receptor.