LG2055 activates the Nrf2-ARE signaling pathway by activating the JNK signaling pathway.

<p>MEF cells were preincubated with 20 μM SP600125 for 1 h and treated with LG2055 for 24 h. (A) The total cell lysates were analyzed by western blotting to compare the Nrf2 protein levels. The relative expression level of Nrf2 normalized by β-actin protein expression was quantitated. (B) The cells were fractionated into cytosolic and nuclear fractions using the Focus SubCell kit. The nuclear fraction was analyzed by western blotting to compare Nrf2 protein levels. The relative expression level of Nrf2 normalized by Lamin B1 protein expression was quantitated. (C) The mRNA expression levels of Nrf2 target genes were determined by quantitative real time-PCR analysis. (A-C) Each experiment was performed in triplicate; the data are shown as the means ± SD. Values not sharing a common letter are significantly different according to one-way ANOVA and Tukey-Kramer post-test analysis; <i>p</i> <0.05.