NgAgo showed no genome editing activity on plasmid and genomic DNA.

<p><b>A</b>. Untagged NgAgo produced green fluorescence positive cells independent of guide DNA. SaCas9, tagged NgAgo or untagged NgAgo were cotransfected with reporter plasmid DNA into HEK293T cells. In SaCas9 transfection single strand guide DNA was included to equate transfection conditions. Scale bar: 100 μm. <b>B</b>. Next-generation sequencing of the target region of reporter plasmid DNA amplified from cells co-transfected with untagged NgAgo and F2+R1 oligos. The most appearing seven types of reads are listed with their percentages. Positions of the guide oligos are highlighted, with direction indicated by arrows. <b>C</b>. Sequencing of the target region of human <i>MYC</i> promoter amplified from cells co-transfected with untagged NgAgo and the indicated oligos. The most appearing seven types of reads are listed with their percentages. Positions of the guide oligos are highlighted, with direction indicated by arrows.