<p><b>A</b>. Schematic diagram of components between the start and second codons of <i>EGFP</i> cDNA in the reporter cell line. A stop codon was included 5’ to the start codon to prevent unexpected translation into the <i>EGFP</i> cDNA from a start codon 5’ to the authentic start codon. A Kozak sequence was included for efficient translation. The inserted 119 bp sequence disrupts the reading frame from the <i>EGFP</i> start codon. Deletion of 3N+2 or insertion of 3N+1 base pairs restore the <i>EGFP</i> reading frame. Positions and directions (arrow head points to the 3’ end) of the single-strand guide DNA for NgAgo experiments are indicated. <b>B</b>. GFP was activated in the presence of SaCas9 and sgRNAs (middle and right) but not without ...
<p>(a) Reporter consists of a HeLa cell line with two stably integrated copies of EGFP with a mutate...
<p><b>A</b>. 3’ RACE analysis of <i>egfp</i> gene transcripts. An oligo (dT) 3’ RACE adapter primer ...
<p>Gene editing in human embryonic stem cells (hESCs) has been significantly enhanced by the discove...
To make the EGFRvIII mutant cell line in H9, gene editing was performed using CRISRP-CAS9 with two s...
<p><b>(A) Allelic analysis of eGFP positive cells expanded as a clonal population.</b> Clonally isol...
<p>Unsynchronized or synchronized and released cells were transfected via electroporation with CRISP...
<p>(A) Sequence analysis of pIREShyg2-EGFP-[AAAG]17 plasmid. Genomic DNA was isolated, the EGFP regi...
(a) Schematic diagram of the TGFBI partial protein coding region and the locus targeted by the sgRNA...
<p>A. Schematic of sequencing primers and areas of interest. Arrows indicate the location of the pri...
<p>The wild-type and mutated eGFP gene segments with the target codon located in the center of the s...
<p>Real-time quantitative reverse transcription PCR analysis of <i>egfp</i>. Viral constructs with o...
<p><b>A</b>. Untagged NgAgo produced green fluorescence positive cells independent of guide DNA. SaC...
<p>(<i>A</i>) Heatmap depicting genomic breakpoints within <i>EGFR</i> in the glioblastoma cell line...
<p>(A) PCR primers (P1–P6) that specifically amplify full length <i>EGFP</i>, Δ3<i>egfp</i>, and Δ5<...
<p>The protein coding sequence (arrow) and the transcription start (broken arrow) are represented ou...
<p>(a) Reporter consists of a HeLa cell line with two stably integrated copies of EGFP with a mutate...
<p><b>A</b>. 3’ RACE analysis of <i>egfp</i> gene transcripts. An oligo (dT) 3’ RACE adapter primer ...
<p>Gene editing in human embryonic stem cells (hESCs) has been significantly enhanced by the discove...
To make the EGFRvIII mutant cell line in H9, gene editing was performed using CRISRP-CAS9 with two s...
<p><b>(A) Allelic analysis of eGFP positive cells expanded as a clonal population.</b> Clonally isol...
<p>Unsynchronized or synchronized and released cells were transfected via electroporation with CRISP...
<p>(A) Sequence analysis of pIREShyg2-EGFP-[AAAG]17 plasmid. Genomic DNA was isolated, the EGFP regi...
(a) Schematic diagram of the TGFBI partial protein coding region and the locus targeted by the sgRNA...
<p>A. Schematic of sequencing primers and areas of interest. Arrows indicate the location of the pri...
<p>The wild-type and mutated eGFP gene segments with the target codon located in the center of the s...
<p>Real-time quantitative reverse transcription PCR analysis of <i>egfp</i>. Viral constructs with o...
<p><b>A</b>. Untagged NgAgo produced green fluorescence positive cells independent of guide DNA. SaC...
<p>(<i>A</i>) Heatmap depicting genomic breakpoints within <i>EGFR</i> in the glioblastoma cell line...
<p>(A) PCR primers (P1–P6) that specifically amplify full length <i>EGFP</i>, Δ3<i>egfp</i>, and Δ5<...
<p>The protein coding sequence (arrow) and the transcription start (broken arrow) are represented ou...
<p>(a) Reporter consists of a HeLa cell line with two stably integrated copies of EGFP with a mutate...
<p><b>A</b>. 3’ RACE analysis of <i>egfp</i> gene transcripts. An oligo (dT) 3’ RACE adapter primer ...
<p>Gene editing in human embryonic stem cells (hESCs) has been significantly enhanced by the discove...