Determination of the interactions between P7-2 and rice OSK proteins by yeast two-hybrid assay.

<p>The growth of yeast cells harboring the P7-2 fused to the GAL4 DNA-binding domain (BD), and OSK proteins fused to the GAL4 activation domain (AD) are shown. SD/WL was the nonselective medium and SD/AHWL/X-α-gal was the selective medium. For serial dilution analysis, yeast cells were collected and adjusted to OD₆₀₀ = 1.0, and diluted to 1, 10⁻¹, 10⁻² and 10⁻³. Corresponding yeast transformants were spotted on SD/AHWL/X-α-gal. Plasmids expressing only the DNA-activation domain or DNA-binding domain were used as negative controls. SD/WL, -Trp-Leu; SD/AHWL/X-α-gal, -Ade-His-Trp-Leu containing X-α-gal.