Overexpression of miR-338-3p decreases <i>in vitro</i> proliferation of GBM cells.

<p>(A) Construction of miR-338-3p overexpressor lentivirus containing a GFP-coding region to indicate expression along with two miR-338-3p transcripts downstream of the U6 promoter. (B) Endogenous expression of miR-338-3p in U251 GBM cells, as indicated by miR-338-3p sensor lentivirus (red) expression compared to control lentivirus expressing GFP-only. (C) Expression of miR-338-3p following infection with overexpressor virus in U251 GBM cells as indicated by miR-338-3p sensor (red). (D) Endogenous expression of miR-338-3p in SF295 GBM cells, as indicated by miR-338-3p sensor lentivirus (red) expression compared to control lentivirus expressing GFP-only. (E) Expression of miR-338-3p following infection with overexpressor virus in and SF295 GBM cells as indicated by miR-338-3p sensor (red). (F) Population growth kinetics of U251 GBM cells infected with an empty vector or miR-338-3p overexpressor (7–12 DPI). (G) Population growth kinetics of SF295 GBM cells infected with an empty vector or miR-338-3p overexpressor (7–10 DPI). Dotted lines in (F) and (G) fit theoretical population growth curves to the observed data, using the equation: Y = 25 × 2^t/DT, where Y is the number of cells at time t, and DT is the doubling time. ****p<0.001; Pearson’s chi-squared test. Results show mean ± SEM.