Effect of 17-AAG on the expression of HSP70 in glioblastoma cells.

<p>(A) U-87 MG cells were treated with increasing concentrations of 17-AAG for 24 h and 48 h or (B) D11 for 24 h. Control cells (CT) were treated with 0.1% DMSO. The expression of the indicated proteins was verified by Western blot analysis. β-actin was detected as loading control. (C) Phase-contrast microscopy pictures of U-87 MG cells treated with vehicle (24 h), 0.5 μM 17-AAG (24 h), 50 μM D11 (5 h or 24 h) or the combination. Combination (1) refers to cells treated with 17-AAG and D11 for 24 h. Combination (2) refers to cells treated with 17-AAG for 24 h and D11 added in the last 5 h of incubation time. Cell pictures were taken at 100x magnification. (D) U-87 MG cells were treated as described in (C). Expression of the indicated proteins was analyzed by Western blot. (E) and (F), M059K cells were treated and analyzed essentially as described in (C) for 24 h. Experiments were performed three times obtaining similar results.